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        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411
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        About MultiQC

        This report was generated using MultiQC, version 1.35

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        MultiQC is developed by Seqera.

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        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        This report has been generated by the STAPLE analysis pipeline.
        Report generated on 2026-10-07, 13:43 EDT based on data in: /local/scratch/dlvovs/work/cf/b350207fe9ce6d630c8080eaec094b

        Sample Sheet

        The sample sheet provided as input to the pipeline. Sample sheet contains sample name, paths to the input data files and associated metadata.

        Showing 6/6 rows and 9/9 columns.
        sampledata_directoryexpression_profilepatientyear_borngendertimepointtimepoint_datesample_typeradiotherapy
        T0-0078011
        /local/projects-t3/fertig_staple/osteosarc/xenium/T0/output-XETG00279__0078011__B3__20260211__182948
        XETG00279
        1979
        male
        T0
        2022-11
        resection
        pre
        T0-0078018
        /local/projects-t3/fertig_staple/osteosarc/xenium/T0/output-XETG00279__0078018__C3__20260211__182947
        XETG00279
        1979
        male
        T0
        2022-11
        resection
        pre
        T1-0102917
        /local/projects-t3/fertig_staple/osteosarc/xenium/20260513__172829__jw_batch2/output-XETG00279__0102917__Region_1__20260513__172840
        XETG00279
        1979
        male
        T1
        2024-06
        re-resection
        pre
        T2-0102913
        /local/projects-t3/fertig_staple/osteosarc/xenium/20260513__172829__jw_batch2/output-XETG00279__0102913__Region_1__20260513__172841
        XETG00279
        1979
        male
        T2
        2025-01
        biopsy
        pre
        T3-0103076
        /local/projects-t3/fertig_staple/osteosarc/xenium/20260515__160744__jw_batch3/output-XETG00279__0103076__Region_1__20260515__160758
        XETG00279
        1979
        male
        T3
        2025-04
        resection
        post
        T3-0103350
        /local/projects-t3/fertig_staple/osteosarc/xenium/20260515__160744__jw_batch3/output-XETG00279__0103350__Region_1__20260515__160758
        XETG00279
        1979
        male
        T3
        2025-04
        resection
        post

        Input Data

        Summaries of the input datasets.

        Input Summary

        Summary of the AnnData object right after it was read.

        Showing 6/6 rows and 6/6 columns.
        Samplen_genesn_cellsmean_genes_by_countsmean_cells_by_countsmean_total_nnz_countsmean_percent_mito
        T0-0078011
        428
        318320
        41.8
        31100.9
        5.0
        0.0
        T0-0078018
        428
        426526
        54.9
        54710.1
        4.7
        0.0
        T1-0102917
        428
        34478
        81.0
        6526.3
        4.1
        0.0
        T2-0102913
        428
        122202
        69.1
        19715.5
        3.0
        0.0
        T3-0103076
        428
        273156
        26.5
        16883.9
        2.7
        0.0
        T3-0103350
        428
        201503
        32.1
        15100.1
        3.0
        0.0

        Reference Cell Types

        Summary of the cell types in the atlas. Provides an overview of the diversity and abundance of cell types used as reference in deconvolution.

        Showing 1/1 rows and 16/16 columns.
        SampleT_Cells_CD8T_Cells_CD4MacrophagesTumorMitoEndothelial_cellsB_cellsMonocytes_classicalNeutrophilsNK_cellsRiboOsteoclastsMacrophages_perivascularcDCsMonocytes_non_classicalpDCs
        072925_IPISRC044_T1_T2_T3_sobj_merged_processed_tcr_bcr_mutMap_tcMap_annot_final_reannot
        9426
        4343
        4198
        3571
        1391
        975
        830
        728
        636
        583
        442
        419
        211
        136
        107
        50

        Reference Summary

        Summary of the cells and genes in the atlas. Atlas is used as a reference for deconvolution and cell type assignment.

        Showing 1/1 rows and 6/6 columns.
        Samplen_genesn_cellsmean_genes_by_countsmean_cells_by_countsmean_total_nnz_countsmean_percent_mito
        072925_IPISRC044_T1_T2_T3_sobj_merged_processed_tcr_bcr_mutMap_tcMap_annot_final_reannot
        29264
        28046
        3030.4
        2904.3
        3.7
        2.2

        Cell Typing

        Summaries of the cell typing results.

        Cell Type Counts

        Assigned cell types based on deconvolution / cell typing results. Unassigned counts appear in a column with no header.

        Showing 6/6 rows and 17/17 columns.
        SampleTumorEndothelial cellsMacrophagesT Cells CD8B cellsOsteoclastsT Cells CD4Monocytes classicalMacrophages perivascularMitocDCsRibopDCsNK cellsMonocytes non classicalNeutrophils
        T0-0078011
        164735
        105697
        25594
        9468
        2567
        1771
        1395
        1291
        1143
        1022
        899
        702
        520
        503
        487
        391
        135
        T0-0078018
        294379
        79307
        23437
        14141
        1283
        182
        702
        1159
        743
        7129
        1956
        657
        939
        60
        259
        99
        94
        T1-0102917
        25179
        4545
        1721
        719
        203
        15
        113
        93
        817
        591
        53
        165
        80
        8
        80
        75
        21
        T2-0102913
        66927
        22905
        2995
        10319
        6108
        2911
        695
        2168
        1596
        899
        838
        2424
        529
        117
        585
        175
        11
        T3-0103076
        63304
        164294
        1779
        16936
        9889
        1148
        2851
        4837
        1990
        1394
        1387
        1132
        1037
        153
        844
        155
        26
        T3-0103350
        61252
        104549
        1283
        13729
        7610
        620
        2263
        3646
        1545
        1014
        1379
        839
        852
        98
        638
        163
        23

        Cell Type Probabilities

        Average max cell type probabilities (fractions) used to assign the cell types. Greater values indicate more homogenous cell type assignments.

        Showing 6/6 rows and 16/16 columns.
        SampleB cellsEndothelial cellsMacrophagesMacrophages perivascularMitoMonocytes classicalMonocytes non classicalNK cellsNeutrophilsOsteoclastsRiboT Cells CD4T Cells CD8TumorcDCspDCs
        T0-0078011
        0.5
        0.7
        0.5
        0.4
        0.4
        0.5
        0.4
        0.5
        0.4
        0.6
        0.5
        0.6
        0.5
        0.8
        0.5
        0.4
        T0-0078018
        0.3
        0.6
        0.4
        0.4
        0.4
        0.4
        0.4
        0.5
        0.6
        0.4
        0.5
        0.5
        0.5
        0.8
        0.4
        0.3
        T1-0102917
        0.3
        0.7
        0.5
        0.4
        0.4
        0.5
        0.5
        0.6
        0.5
        0.4
        0.5
        0.6
        0.6
        0.8
        0.4
        0.3
        T2-0102913
        0.5
        0.7
        0.5
        0.4
        0.4
        0.5
        0.4
        0.4
        0.3
        0.4
        0.4
        0.5
        0.5
        0.8
        0.5
        0.5
        T3-0103076
        0.5
        0.7
        0.6
        0.5
        0.5
        0.5
        0.6
        0.5
        0.4
        0.5
        0.4
        0.5
        0.6
        0.8
        0.6
        0.6
        T3-0103350
        0.5
        0.7
        0.6
        0.5
        0.5
        0.5
        0.5
        0.5
        0.3
        0.5
        0.4
        0.5
        0.5
        0.8
        0.6
        0.6

        Graph Stats

        This graph measure is the fraction of non-group members connected to group members

        Average Clustering

        This graph measure shows the degree to which nodes cluster together.

        Showing 6/6 rows and 17/17 columns.
        Sample NameB cellsEndothelial cellsMacrophagesMacrophages perivascularMitoMonocytes classicalMonocytes non classicalNK cellsNeutrophilsOsteoclastsRiboT Cells CD4T Cells CD8TumorcDCspDCsNA
        T0-0078011
        0.4
        0.5
        0.4
        0.4
        0.5
        0.4
        0.3
        0.5
        0.4
        0.4
        0.5
        0.5
        0.5
        0.4
        0.5
        0.4
        0.4
        T0-0078018
        0.5
        0.5
        0.5
        0.5
        0.5
        0.5
        0.5
        0.5
        0.4
        0.5
        0.5
        0.5
        0.5
        0.4
        0.5
        0.5
        0.5
        T1-0102917
        0.3
        0.4
        0.4
        0.4
        0.5
        0.3
        0.4
        0.3
        0.3
        0.3
        0.4
        0.4
        0.4
        0.4
        0.4
        0.5
        0.4
        T2-0102913
        0.4
        0.4
        0.4
        0.4
        0.4
        0.4
        0.5
        0.4
        0.4
        0.5
        0.4
        0.4
        0.4
        0.4
        0.4
        0.4
        0.5
        T3-0103076
        0.4
        0.4
        0.4
        0.4
        0.4
        0.4
        0.3
        0.4
        0.3
        0.4
        0.4
        0.4
        0.4
        0.4
        0.4
        0.4
        0.3
        T3-0103350
        0.4
        0.4
        0.4
        0.4
        0.4
        0.4
        0.3
        0.4
        0.4
        0.4
        0.4
        0.4
        0.4
        0.4
        0.4
        0.4
        0.3

        Closeness Centrality

        This graph measure reflects how close the group is to other nodes.

        Showing 6/6 rows and 17/17 columns.
        Sample NameB cellsEndothelial cellsMacrophagesMacrophages perivascularMitoMonocytes classicalMonocytes non classicalNK cellsNeutrophilsOsteoclastsRiboT Cells CD4T Cells CD8TumorcDCspDCsNA
        T0-0078011
        0.0
        0.4
        0.3
        0.1
        0.1
        0.1
        0.0
        0.1
        0.0
        0.1
        0.1
        0.1
        0.1
        0.6
        0.1
        0.0
        0.4
        T0-0078018
        0.0
        0.3
        0.3
        0.2
        0.1
        0.1
        0.0
        0.0
        0.0
        0.0
        0.1
        0.1
        0.1
        0.8
        0.1
        0.0
        0.3
        T1-0102917
        0.0
        0.4
        0.2
        0.2
        0.1
        0.2
        0.0
        0.1
        0.4
        0.1
        0.1
        0.1
        0.1
        1.4
        0.1
        0.0
        0.2
        T2-0102913
        0.1
        0.2
        0.3
        0.1
        0.1
        0.1
        0.0
        0.1
        0.0
        0.1
        0.1
        0.2
        0.3
        0.3
        0.2
        0.0
        0.3
        T3-0103076
        0.2
        0.3
        0.5
        0.2
        0.2
        0.2
        0.1
        0.3
        0.1
        0.3
        0.3
        0.5
        0.4
        0.4
        0.2
        0.1
        0.5
        T3-0103350
        0.2
        0.2
        0.5
        0.2
        0.2
        0.2
        0.1
        0.2
        0.0
        0.3
        0.2
        0.3
        0.5
        0.7
        0.2
        0.1
        0.4

        Degree Centrality

        This graph measure is the fraction of non-group members connected to group members

        Showing 6/6 rows and 17/17 columns.
        Sample NameB cellsEndothelial cellsMacrophagesMacrophages perivascularMitoMonocytes classicalMonocytes non classicalNK cellsNeutrophilsOsteoclastsRiboT Cells CD4T Cells CD8TumorcDCspDCsNA
        T0-0078011
        0.0
        0.2
        0.1
        0.0
        0.0
        0.0
        0.0
        0.0
        0.0
        0.0
        0.0
        0.0
        0.0
        0.4
        0.0
        0.0
        0.3
        T0-0078018
        0.0
        0.2
        0.1
        0.1
        0.0
        0.0
        0.0
        0.0
        0.0
        0.0
        0.0
        0.0
        0.0
        0.7
        0.0
        0.0
        0.2
        T1-0102917
        0.0
        0.2
        0.1
        0.1
        0.0
        0.1
        0.0
        0.0
        0.0
        0.0
        0.0
        0.0
        0.0
        0.5
        0.0
        0.0
        0.1
        T2-0102913
        0.1
        0.1
        0.2
        0.0
        0.0
        0.1
        0.0
        0.0
        0.0
        0.0
        0.0
        0.1
        0.2
        0.4
        0.1
        0.0
        0.2
        T3-0103076
        0.0
        0.0
        0.2
        0.0
        0.0
        0.0
        0.0
        0.0
        0.0
        0.1
        0.0
        0.1
        0.2
        0.2
        0.0
        0.0
        0.5
        T3-0103350
        0.0
        0.0
        0.2
        0.0
        0.0
        0.0
        0.0
        0.0
        0.0
        0.1
        0.0
        0.1
        0.2
        0.3
        0.0
        0.0
        0.4

        Spatial Neighbors

        Cell type neighborhood across samples. The rate of self-neighborhood indicates clustering of a cell type. The rate of neighbors with other cell types (self omitted) indicates how these clusters interact with each other. Self is omitted in this report based on the pipeline settings.

        Created with MultiQC

        Moran I By Radiotherapy

        Top 50 differential scores across samples. There are 20 significant differential scores (p_adj<=0.05) found between ['T3-0103350', 'T3-0103076'] and ['T1-0102917', 'T2-0102913', 'T0-0078011', 'T0-0078018'], marked with *.

        Created with MultiQC

        Co Occurrence By Radiotherapy

        Median difference of co-occurrence across groups by variable radiotherapy (post - pre). The plot is organized by focal cell type and displays differences in co-occurrence of each other cell type with the focal cell type at each of the observed intervals. Spotlight mode on ['Tumor'].

        Created with MultiQC

        Deseq2 By Radiotherapy

        DESeq2 results for radiotherapy variable with significant DE genes (padj<=0.05) between post and pre. Log2 fold change (X) and -log10 adjusted p-value (Y) shown.

        Created with MultiQC

        Neighbors By Radiotherapy

        Neighbors By Radiotherapy cell_type-neighbors by radiotherapy: t-test on ALR normalized counts relative to self-neighbors. Spotlight on ['Tumor']. There are 0 significant (p_adj<=0.05) results marked with *.

        Showing 6/6 rows and 16/16 columns.
        Sample NameTumor-OsteoclastsTumor-Endothelial cellsTumor-NK cellsTumor-MacrophagesTumor-T Cells CD4Tumor-T Cells CD8Tumor-RiboTumor-MitoTumor-NATumor-B cellsTumor-NeutrophilsTumor-Monocytes classicalTumor-cDCsTumor-Monocytes non classicalTumor-pDCsTumor-Macrophages perivascular
        T0-0078011
        -5.2
        -2.5
        -7.0
        -3.1
        -6.1
        -5.5
        -6.8
        -5.7
        -2.4
        -6.9
        -8.8
        -6.6
        -6.3
        -8.4
        -7.5
        -6.3
        T0-0078018
        -6.1
        -2.7
        -7.2
        -3.1
        -5.6
        -5.6
        -5.8
        -5.0
        -2.5
        -7.6
        -9.1
        -6.1
        -6.3
        -8.5
        -8.7
        -3.8
        T1-0102917
        -6.0
        -2.8
        -6.5
        -3.9
        -6.0
        -5.0
        -6.0
        -6.2
        -3.7
        -7.8
        -10.9
        -4.2
        -5.4
        -7.3
        -8.3
        -3.9
        T2-0102913
        -6.1
        -3.8
        -6.3
        -3.0
        -4.5
        -3.7
        -5.8
        -5.0
        -3.3
        -4.8
        -11.0
        -4.6
        -4.3
        -7.7
        -7.9
        -6.1
        T3-0103076
        -3.7
        -4.4
        -5.2
        -1.9
        -3.3
        -2.5
        -4.8
        -4.3
        -1.7
        -4.9
        -8.8
        -4.5
        -5.0
        -7.5
        -7.4
        -4.5
        T3-0103350
        -3.9
        -4.6
        -5.3
        -2.0
        -3.4
        -2.6
        -4.9
        -4.3
        -1.9
        -5.4
        -8.7
        -4.6
        -5.1
        -7.7
        -8.1
        -4.8

        Squidpy Ligrec By Radiotherapy

        Top 50 differential scores across samples. There are 4 significant differential scores (p_adj<=0.05) found between ['T3-0103350', 'T3-0103076'] and ['T1-0102917', 'T2-0102913', 'T0-0078011', 'T0-0078018'], marked with *.

        Created with MultiQC

        Software Versions

        Software Versions lists versions of software tools extracted from file contents.

        GroupSoftwareVersion
        ADATA_ADD_METADATAanndata0.12.11
        ADATA_FROM_XENIUMspatialdata_io0.6.0
        squidpy1.8.1
        ADATA_PREPROCESSanndata0.12.11
        ATLAS_GETboto31.42.93
        requests2.33.1
        ATLAS_MATCHanndata0.12.11
        numpy2.4.4
        QCanndata0.12.11
        pandas2.3.3
        RCTDMatrix1.7-4
        R4.5.3
        reticulate1.45.0
        spacexr2.2.1
        SQUIDPY_LIGREC_ANALYSISanndata0.12.10
        scanpy1.11.5
        squidpy1.6.6.dev24+g32789aefd
        SQUIDPY_SPATIALanndata0.12.10
        numpy2.4.3
        python3.11.15
        squidpy1.6.6.dev24+g32789aefd
        STAPLE_XSAMPLEanndata0.12.10
        matplotlib3.10.8
        numpy2.4.3
        pandas2.3.3
        pydeseq20.5.4
        scipy1.17.1
        seaborn0.13.2
        WorkflowNextflow26.04.1
        STAPLEv2.5.0-g8ae233d