A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.
This report has been generated by the STAPLE analysis pipeline.
/local/scratch/dlvovs/work/cf/b350207fe9ce6d630c8080eaec094b
Sample Sheet
The sample sheet provided as input to the pipeline. Sample sheet contains sample name, paths to the input data files and associated metadata.
| sample | data_directory | expression_profile | patient | year_born | gender | timepoint | timepoint_date | sample_type | radiotherapy |
|---|---|---|---|---|---|---|---|---|---|
| T0-0078011 | /local/projects-t3/fertig_staple/osteosarc/xenium/T0/output-XETG00279__0078011__B3__20260211__182948 | XETG00279 | 1979 | male | T0 | 2022-11 | resection | pre | |
| T0-0078018 | /local/projects-t3/fertig_staple/osteosarc/xenium/T0/output-XETG00279__0078018__C3__20260211__182947 | XETG00279 | 1979 | male | T0 | 2022-11 | resection | pre | |
| T1-0102917 | /local/projects-t3/fertig_staple/osteosarc/xenium/20260513__172829__jw_batch2/output-XETG00279__0102917__Region_1__20260513__172840 | XETG00279 | 1979 | male | T1 | 2024-06 | re-resection | pre | |
| T2-0102913 | /local/projects-t3/fertig_staple/osteosarc/xenium/20260513__172829__jw_batch2/output-XETG00279__0102913__Region_1__20260513__172841 | XETG00279 | 1979 | male | T2 | 2025-01 | biopsy | pre | |
| T3-0103076 | /local/projects-t3/fertig_staple/osteosarc/xenium/20260515__160744__jw_batch3/output-XETG00279__0103076__Region_1__20260515__160758 | XETG00279 | 1979 | male | T3 | 2025-04 | resection | post | |
| T3-0103350 | /local/projects-t3/fertig_staple/osteosarc/xenium/20260515__160744__jw_batch3/output-XETG00279__0103350__Region_1__20260515__160758 | XETG00279 | 1979 | male | T3 | 2025-04 | resection | post |
Input Data
Summaries of the input datasets.
Input Summary
Summary of the AnnData object right after it was read.
| Sample | n_genes | n_cells | mean_genes_by_counts | mean_cells_by_counts | mean_total_nnz_counts | mean_percent_mito |
|---|---|---|---|---|---|---|
| T0-0078011 | 428 | 318320 | 41.8 | 31100.9 | 5.0 | 0.0 |
| T0-0078018 | 428 | 426526 | 54.9 | 54710.1 | 4.7 | 0.0 |
| T1-0102917 | 428 | 34478 | 81.0 | 6526.3 | 4.1 | 0.0 |
| T2-0102913 | 428 | 122202 | 69.1 | 19715.5 | 3.0 | 0.0 |
| T3-0103076 | 428 | 273156 | 26.5 | 16883.9 | 2.7 | 0.0 |
| T3-0103350 | 428 | 201503 | 32.1 | 15100.1 | 3.0 | 0.0 |
Reference Cell Types
Summary of the cell types in the atlas. Provides an overview of the diversity and abundance of cell types used as reference in deconvolution.
| Sample | T_Cells_CD8 | T_Cells_CD4 | Macrophages | Tumor | Mito | Endothelial_cells | B_cells | Monocytes_classical | Neutrophils | NK_cells | Ribo | Osteoclasts | Macrophages_perivascular | cDCs | Monocytes_non_classical | pDCs |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 072925_IPISRC044_T1_T2_T3_sobj_merged_processed_tcr_bcr_mutMap_tcMap_annot_final_reannot | 9426 | 4343 | 4198 | 3571 | 1391 | 975 | 830 | 728 | 636 | 583 | 442 | 419 | 211 | 136 | 107 | 50 |
Reference Summary
Summary of the cells and genes in the atlas. Atlas is used as a reference for deconvolution and cell type assignment.
| Sample | n_genes | n_cells | mean_genes_by_counts | mean_cells_by_counts | mean_total_nnz_counts | mean_percent_mito |
|---|---|---|---|---|---|---|
| 072925_IPISRC044_T1_T2_T3_sobj_merged_processed_tcr_bcr_mutMap_tcMap_annot_final_reannot | 29264 | 28046 | 3030.4 | 2904.3 | 3.7 | 2.2 |
Cell Typing
Summaries of the cell typing results.
Cell Type Counts
Assigned cell types based on deconvolution / cell typing results. Unassigned counts appear in a column with no header.
| Sample | Tumor | Endothelial cells | Macrophages | T Cells CD8 | B cells | Osteoclasts | T Cells CD4 | Monocytes classical | Macrophages perivascular | Mito | cDCs | Ribo | pDCs | NK cells | Monocytes non classical | Neutrophils | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| T0-0078011 | 164735 | 105697 | 25594 | 9468 | 2567 | 1771 | 1395 | 1291 | 1143 | 1022 | 899 | 702 | 520 | 503 | 487 | 391 | 135 |
| T0-0078018 | 294379 | 79307 | 23437 | 14141 | 1283 | 182 | 702 | 1159 | 743 | 7129 | 1956 | 657 | 939 | 60 | 259 | 99 | 94 |
| T1-0102917 | 25179 | 4545 | 1721 | 719 | 203 | 15 | 113 | 93 | 817 | 591 | 53 | 165 | 80 | 8 | 80 | 75 | 21 |
| T2-0102913 | 66927 | 22905 | 2995 | 10319 | 6108 | 2911 | 695 | 2168 | 1596 | 899 | 838 | 2424 | 529 | 117 | 585 | 175 | 11 |
| T3-0103076 | 63304 | 164294 | 1779 | 16936 | 9889 | 1148 | 2851 | 4837 | 1990 | 1394 | 1387 | 1132 | 1037 | 153 | 844 | 155 | 26 |
| T3-0103350 | 61252 | 104549 | 1283 | 13729 | 7610 | 620 | 2263 | 3646 | 1545 | 1014 | 1379 | 839 | 852 | 98 | 638 | 163 | 23 |
Cell Type Probabilities
Average max cell type probabilities (fractions) used to assign the cell types. Greater values indicate more homogenous cell type assignments.
| Sample | B cells | Endothelial cells | Macrophages | Macrophages perivascular | Mito | Monocytes classical | Monocytes non classical | NK cells | Neutrophils | Osteoclasts | Ribo | T Cells CD4 | T Cells CD8 | Tumor | cDCs | pDCs |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| T0-0078011 | 0.5 | 0.7 | 0.5 | 0.4 | 0.4 | 0.5 | 0.4 | 0.5 | 0.4 | 0.6 | 0.5 | 0.6 | 0.5 | 0.8 | 0.5 | 0.4 |
| T0-0078018 | 0.3 | 0.6 | 0.4 | 0.4 | 0.4 | 0.4 | 0.4 | 0.5 | 0.6 | 0.4 | 0.5 | 0.5 | 0.5 | 0.8 | 0.4 | 0.3 |
| T1-0102917 | 0.3 | 0.7 | 0.5 | 0.4 | 0.4 | 0.5 | 0.5 | 0.6 | 0.5 | 0.4 | 0.5 | 0.6 | 0.6 | 0.8 | 0.4 | 0.3 |
| T2-0102913 | 0.5 | 0.7 | 0.5 | 0.4 | 0.4 | 0.5 | 0.4 | 0.4 | 0.3 | 0.4 | 0.4 | 0.5 | 0.5 | 0.8 | 0.5 | 0.5 |
| T3-0103076 | 0.5 | 0.7 | 0.6 | 0.5 | 0.5 | 0.5 | 0.6 | 0.5 | 0.4 | 0.5 | 0.4 | 0.5 | 0.6 | 0.8 | 0.6 | 0.6 |
| T3-0103350 | 0.5 | 0.7 | 0.6 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.3 | 0.5 | 0.4 | 0.5 | 0.5 | 0.8 | 0.6 | 0.6 |
Graph Stats
This graph measure is the fraction of non-group members connected to group members
Average Clustering
This graph measure shows the degree to which nodes cluster together.
| Sample Name | B cells | Endothelial cells | Macrophages | Macrophages perivascular | Mito | Monocytes classical | Monocytes non classical | NK cells | Neutrophils | Osteoclasts | Ribo | T Cells CD4 | T Cells CD8 | Tumor | cDCs | pDCs | NA |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| T0-0078011 | 0.4 | 0.5 | 0.4 | 0.4 | 0.5 | 0.4 | 0.3 | 0.5 | 0.4 | 0.4 | 0.5 | 0.5 | 0.5 | 0.4 | 0.5 | 0.4 | 0.4 |
| T0-0078018 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.4 | 0.5 | 0.5 | 0.5 | 0.5 | 0.4 | 0.5 | 0.5 | 0.5 |
| T1-0102917 | 0.3 | 0.4 | 0.4 | 0.4 | 0.5 | 0.3 | 0.4 | 0.3 | 0.3 | 0.3 | 0.4 | 0.4 | 0.4 | 0.4 | 0.4 | 0.5 | 0.4 |
| T2-0102913 | 0.4 | 0.4 | 0.4 | 0.4 | 0.4 | 0.4 | 0.5 | 0.4 | 0.4 | 0.5 | 0.4 | 0.4 | 0.4 | 0.4 | 0.4 | 0.4 | 0.5 |
| T3-0103076 | 0.4 | 0.4 | 0.4 | 0.4 | 0.4 | 0.4 | 0.3 | 0.4 | 0.3 | 0.4 | 0.4 | 0.4 | 0.4 | 0.4 | 0.4 | 0.4 | 0.3 |
| T3-0103350 | 0.4 | 0.4 | 0.4 | 0.4 | 0.4 | 0.4 | 0.3 | 0.4 | 0.4 | 0.4 | 0.4 | 0.4 | 0.4 | 0.4 | 0.4 | 0.4 | 0.3 |
Closeness Centrality
This graph measure reflects how close the group is to other nodes.
| Sample Name | B cells | Endothelial cells | Macrophages | Macrophages perivascular | Mito | Monocytes classical | Monocytes non classical | NK cells | Neutrophils | Osteoclasts | Ribo | T Cells CD4 | T Cells CD8 | Tumor | cDCs | pDCs | NA |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| T0-0078011 | 0.0 | 0.4 | 0.3 | 0.1 | 0.1 | 0.1 | 0.0 | 0.1 | 0.0 | 0.1 | 0.1 | 0.1 | 0.1 | 0.6 | 0.1 | 0.0 | 0.4 |
| T0-0078018 | 0.0 | 0.3 | 0.3 | 0.2 | 0.1 | 0.1 | 0.0 | 0.0 | 0.0 | 0.0 | 0.1 | 0.1 | 0.1 | 0.8 | 0.1 | 0.0 | 0.3 |
| T1-0102917 | 0.0 | 0.4 | 0.2 | 0.2 | 0.1 | 0.2 | 0.0 | 0.1 | 0.4 | 0.1 | 0.1 | 0.1 | 0.1 | 1.4 | 0.1 | 0.0 | 0.2 |
| T2-0102913 | 0.1 | 0.2 | 0.3 | 0.1 | 0.1 | 0.1 | 0.0 | 0.1 | 0.0 | 0.1 | 0.1 | 0.2 | 0.3 | 0.3 | 0.2 | 0.0 | 0.3 |
| T3-0103076 | 0.2 | 0.3 | 0.5 | 0.2 | 0.2 | 0.2 | 0.1 | 0.3 | 0.1 | 0.3 | 0.3 | 0.5 | 0.4 | 0.4 | 0.2 | 0.1 | 0.5 |
| T3-0103350 | 0.2 | 0.2 | 0.5 | 0.2 | 0.2 | 0.2 | 0.1 | 0.2 | 0.0 | 0.3 | 0.2 | 0.3 | 0.5 | 0.7 | 0.2 | 0.1 | 0.4 |
Degree Centrality
This graph measure is the fraction of non-group members connected to group members
| Sample Name | B cells | Endothelial cells | Macrophages | Macrophages perivascular | Mito | Monocytes classical | Monocytes non classical | NK cells | Neutrophils | Osteoclasts | Ribo | T Cells CD4 | T Cells CD8 | Tumor | cDCs | pDCs | NA |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| T0-0078011 | 0.0 | 0.2 | 0.1 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.4 | 0.0 | 0.0 | 0.3 |
| T0-0078018 | 0.0 | 0.2 | 0.1 | 0.1 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.7 | 0.0 | 0.0 | 0.2 |
| T1-0102917 | 0.0 | 0.2 | 0.1 | 0.1 | 0.0 | 0.1 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.5 | 0.0 | 0.0 | 0.1 |
| T2-0102913 | 0.1 | 0.1 | 0.2 | 0.0 | 0.0 | 0.1 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.1 | 0.2 | 0.4 | 0.1 | 0.0 | 0.2 |
| T3-0103076 | 0.0 | 0.0 | 0.2 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.1 | 0.0 | 0.1 | 0.2 | 0.2 | 0.0 | 0.0 | 0.5 |
| T3-0103350 | 0.0 | 0.0 | 0.2 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.1 | 0.0 | 0.1 | 0.2 | 0.3 | 0.0 | 0.0 | 0.4 |
Spatial Neighbors
Cell type neighborhood across samples. The rate of self-neighborhood indicates clustering of a cell type. The rate of neighbors with other cell types (self omitted) indicates how these clusters interact with each other. Self is omitted in this report based on the pipeline settings.
Moran I By Radiotherapy
Top 50 differential scores across samples. There are 20 significant differential scores (p_adj<=0.05) found between ['T3-0103350', 'T3-0103076'] and ['T1-0102917', 'T2-0102913', 'T0-0078011', 'T0-0078018'], marked with *.
Co Occurrence By Radiotherapy
Median difference of co-occurrence across groups by variable radiotherapy (post - pre). The plot is organized by focal cell type and displays differences in co-occurrence of each other cell type with the focal cell type at each of the observed intervals. Spotlight mode on ['Tumor'].
Deseq2 By Radiotherapy
DESeq2 results for radiotherapy variable with significant DE genes (padj<=0.05) between post and pre. Log2 fold change (X) and -log10 adjusted p-value (Y) shown.
Neighbors By Radiotherapy
Neighbors By Radiotherapy cell_type-neighbors by radiotherapy: t-test on ALR normalized counts relative to self-neighbors. Spotlight on ['Tumor']. There are 0 significant (p_adj<=0.05) results marked with *.
| Sample Name | Tumor-Osteoclasts | Tumor-Endothelial cells | Tumor-NK cells | Tumor-Macrophages | Tumor-T Cells CD4 | Tumor-T Cells CD8 | Tumor-Ribo | Tumor-Mito | Tumor-NA | Tumor-B cells | Tumor-Neutrophils | Tumor-Monocytes classical | Tumor-cDCs | Tumor-Monocytes non classical | Tumor-pDCs | Tumor-Macrophages perivascular |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| T0-0078011 | -5.2 | -2.5 | -7.0 | -3.1 | -6.1 | -5.5 | -6.8 | -5.7 | -2.4 | -6.9 | -8.8 | -6.6 | -6.3 | -8.4 | -7.5 | -6.3 |
| T0-0078018 | -6.1 | -2.7 | -7.2 | -3.1 | -5.6 | -5.6 | -5.8 | -5.0 | -2.5 | -7.6 | -9.1 | -6.1 | -6.3 | -8.5 | -8.7 | -3.8 |
| T1-0102917 | -6.0 | -2.8 | -6.5 | -3.9 | -6.0 | -5.0 | -6.0 | -6.2 | -3.7 | -7.8 | -10.9 | -4.2 | -5.4 | -7.3 | -8.3 | -3.9 |
| T2-0102913 | -6.1 | -3.8 | -6.3 | -3.0 | -4.5 | -3.7 | -5.8 | -5.0 | -3.3 | -4.8 | -11.0 | -4.6 | -4.3 | -7.7 | -7.9 | -6.1 |
| T3-0103076 | -3.7 | -4.4 | -5.2 | -1.9 | -3.3 | -2.5 | -4.8 | -4.3 | -1.7 | -4.9 | -8.8 | -4.5 | -5.0 | -7.5 | -7.4 | -4.5 |
| T3-0103350 | -3.9 | -4.6 | -5.3 | -2.0 | -3.4 | -2.6 | -4.9 | -4.3 | -1.9 | -5.4 | -8.7 | -4.6 | -5.1 | -7.7 | -8.1 | -4.8 |
Squidpy Ligrec By Radiotherapy
Top 50 differential scores across samples. There are 4 significant differential scores (p_adj<=0.05) found between ['T3-0103350', 'T3-0103076'] and ['T1-0102917', 'T2-0102913', 'T0-0078011', 'T0-0078018'], marked with *.
Software Versions
Software Versions lists versions of software tools extracted from file contents.
| Group | Software | Version |
|---|---|---|
| ADATA_ADD_METADATA | anndata | 0.12.11 |
| ADATA_FROM_XENIUM | spatialdata_io | 0.6.0 |
| squidpy | 1.8.1 | |
| ADATA_PREPROCESS | anndata | 0.12.11 |
| ATLAS_GET | boto3 | 1.42.93 |
| requests | 2.33.1 | |
| ATLAS_MATCH | anndata | 0.12.11 |
| numpy | 2.4.4 | |
| QC | anndata | 0.12.11 |
| pandas | 2.3.3 | |
| RCTD | Matrix | 1.7-4 |
| R | 4.5.3 | |
| reticulate | 1.45.0 | |
| spacexr | 2.2.1 | |
| SQUIDPY_LIGREC_ANALYSIS | anndata | 0.12.10 |
| scanpy | 1.11.5 | |
| squidpy | 1.6.6.dev24+g32789aefd | |
| SQUIDPY_SPATIAL | anndata | 0.12.10 |
| numpy | 2.4.3 | |
| python | 3.11.15 | |
| squidpy | 1.6.6.dev24+g32789aefd | |
| STAPLE_XSAMPLE | anndata | 0.12.10 |
| matplotlib | 3.10.8 | |
| numpy | 2.4.3 | |
| pandas | 2.3.3 | |
| pydeseq2 | 0.5.4 | |
| scipy | 1.17.1 | |
| seaborn | 0.13.2 | |
| Workflow | Nextflow | 26.04.1 |
| STAPLE | v2.5.0-g8ae233d |